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Understanding the Mechano-Biological Pathways Involved in Bioprinted Skin Remodeling

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title
Understanding the Mechano-Biological Pathways Involved in Bioprinted Skin Remodeling
author
Anis, Sara
abstract
Purpose/Objectives: Uncontrolled reorganization and realignment of 3D bioprinted hydrogel microstructures could lead to undesired shape and structural changes to the printed construct. Reorganization is the first stage of the matrix remodeling process and takes place over the first few hours to days. Fibrin is a popular component of many bioinks and a provisional component of the extracellular matrix present during wound healing and has been used to 3D bioprint skin. Structural remodeling of fibrin networks due to cellular traction forces is a complex process, in part due to the fact that fibrin networks are not linearly elastic, exhibiting strain-stiffening when subjected to large deformations. Cells embedded in fibrin gels attach and exert traction forces to realign and/or degrade the fibrin fibers, and sense and respond to the surrounding substrate in a mechanical feedback loop. Such cellular actions may remodel the printed construct in such a way, rendering it no longer feasible for implantation. The goal of this study is to understand the maturation process of fibrin based bioprinted constructs to better engineer a 3D bioprinted skin.
subject
3D printing
design of experiments
fibroblasts
Mechanical stimulation
reorganization
skin
contributor
Atala, Anthony J (committee chair)
Soker, Shay (committee member)
Lee, Sang Jin (committee member)
date
2022-05-24T08:35:52Z (accessioned)
2022 (issued)
degree
Biomedical Engineering (discipline)
embargo
2027-05-14 (terms)
2027-05-14 (liftdate)
identifier
http://hdl.handle.net/10339/100722 (uri)
language
en (iso)
publisher
Wake Forest University
type
Thesis

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