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Dissecting Lipid Metabolism in Head and Neck Cancer

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title
Dissecting Lipid Metabolism in Head and Neck Cancer
author
Kaminski, Anna Sophia
abstract
Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous disease with the potential to greatly impact quality of life. HNSCC is distinguished by a number of changes in metabolism which contribute to a malignant phenotype. Alterations in lipid and redox metabolism were investigated on a diverse panel of HNSCC cell lines including SCC-61, rSCC-61, JHU-022, FaDu, SQ-20B and SCC-90. The mesenchymal phenotype of SCC-61 imparts unique redox and lipidomic properties that distinguishes it from the other epithelial cell lines in this study. Analysis of untreated HNSCC cells show that larger quantities of lipid rafts are associated with increased sensitivity to radiation, in alignment with previous data. Further investigation was done using lovastatin to determine the effects that statins have on overall lipid and redox metabolism in the HNSCC cell panel. With the exception of rSCC-61, the data indicates that radiation resistant cells have increased sensitivity to statins. Additionally, lovastatin treatment rendered no significant change in lipid rafts, but did cause a significant increase in mitochondrial ROS. This suggests that the sensitivity to statins in this panel of HNSCC cell lines is likely due to the inability of mitochondrial antioxidant systems to cope with the depletion of CoQ10.
subject
cholesterol
HNSCC
lipidomics
statin
contributor
Furdui, Cristina M (committee chair)
Soto-Pantoja, David (committee member)
Ming, Xin (committee member)
date
2022-07-11T19:17:39Z (accessioned)
2022 (issued)
degree
Biomedical Science – MS (discipline)
embargo
2027-05-14 (terms)
2027-05-14 (liftdate)
identifier
http://hdl.handle.net/10339/101021 (uri)
language
en (iso)
publisher
Wake Forest University
type
Thesis

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