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IL6 Transsignaling in Alzheimer's Disease

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title
IL6 Transsignaling in Alzheimer's Disease
author
Quillen, Daniel Joshua
abstract
Alzheimer’s Disease (AD) is a neurodegenerative disease process manifesting clinically with cognitive impairment and dementia (63). While AD pathology is complicated neuroinflammation has been shown as a consistent feature (35). IL6 is a multifaceted cytokine involved in a plethora of cellular mechanisms including both anti-inflammatory and inflammatory processes (1,2). IL6 can signal classically through the membrane bound receptor or by transsignaling forming a complex with the soluble IL6 receptor (sIL6R) and activating membrane bound glycoprotein 130 (gp130) (13-16). Transsignaling has been hypothesized to be the primary mechanism of IL6 mediated neurodegeneration (19). The IL6R rs2228145 coding polymorphism (Asp358Ala) has been demonstrated to increase the levels of sIL6R in both plasma and CSF (56). To determine whether IL6 transsignaling could have a potential role in AD, we genotyped the Ala358 allele and assayed IL6 and sIL6R concentrations in paired samples of plasma and CSF obtained from 120 participants with normal cognition, mild cognitive impairment, or probable AD enrolled in the Wake Forest Alzheimer’s Disease Research Center’s Clinical Core. Inheritance of the Ala358 variant and measures of plasma IL6 and sIL6R were correlated with cognitive status as well as clinical data, including the: Montreal Cognitive Assessment (MoCA), modified Preclinical Alzheimer’s Cognitive Composite (mPACC), cognitive domain scores obtained from the Uniform Data Set (UDSv3), CSF concentrations of pTau 181, β-amyloid (Aβ) Aβ40 and Aβ42 concentrations to determine transsignaling effect on AD Severity. We found that inheritance of one or two copies of the IL6R Ala358 allele and elevated sIL6R levels in plasma and CSF were correlated with poorer scores on mPACC, MoCA and memory domain scores, increases in CSF pTau and decreases in CSF Aβ42/40 ratio in both unadjusted and covariate adjusted statistical models. These data suggest that IL6 transsignaling and the possession of the IL6R Ala358 allele may be modifiers of AD. Follow-up, prospective studies are necessary, as this subgroup of patients may be identified as ideally responsive to IL6 receptor–blocking therapies.
subject
Alzheimer's Disease
IL6
Neurodegeneration
Transsignaling
contributor
Milligan, Carol (committee chair)
Hawkins, Gregory (committee member)
Hughes, Timothy (committee member)
date
2022-07-11T19:17:40Z (accessioned)
2024-05-23T08:30:08Z (available)
2022 (issued)
degree
Neuroscience – MS (discipline)
embargo
2024-05-23 (terms)
identifier
http://hdl.handle.net/10339/101023 (uri)
language
en (iso)
publisher
Wake Forest University
type
Thesis

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