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SYNTHESIS OF NOVEL 3D MICROENVIRONMENT USING ECM-ENHANCED COLLAGEN-PEG-A HYDROGEL

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abstract
The causes of the cellular dysfunction that impedes tissue regeneration are not well understood and are often multi-factorial but are associated with major tissue trauma such as in volumetric muscle loss (VML). Outcomes vary in severity; however, these conditions can lead to loss of limb function, deformity, and disability. There are relatively few reliable models to research such a disorder and fewer treatments available to overcome loss of function. Bioengineering has several promising avenues for treatments through tissue specific biomaterial development such as incorporating key micro-environmental and mechanical properties necessary for normal cellular function. In this thesis we developed a hydrogel augmented with extracellular matrix and seeded with adipose derived stem-like cells (ADCs) to provide the groundwork for a regenerative medicine approach utilizing a cell type found in the periphery of skeletal muscles. We hypothesize that incorporation of decellularized extracellular matrix (ECM) into collagen-poly (ethylene-glycol)-acrylate (CPA) hydrogels will provide a stable and survivable environment for ADCs.CPA, and Collagen-Peg-A-ECM (CEPA) hydrogels were assessed via rheology and exhibited varying levels of structural rigidity and storage modulus was modeled using a multiple linear regression (MLR) model and an additive model. ADCs encapsulated in both CPA and CEPA hydrogels showed differences in cell viability.
subject
3D Microenvironment
Collagen
Hydrogel
Polyethylene Glycol
contributor
Mischo, Seth H (author)
Soker, Shay (committee chair)
Rahbar, Elaheh (committee member)
Criswell, Tracy (committee member)
date
2022-07-11T19:17:47Z (accessioned)
2022-07-11T19:17:47Z (available)
2022 (issued)
degree
Biomedical Engineering (discipline)
identifier
http://hdl.handle.net/10339/101033 (uri)
language
en (iso)
publisher
Wake Forest University
title
SYNTHESIS OF NOVEL 3D MICROENVIRONMENT USING ECM-ENHANCED COLLAGEN-PEG-A HYDROGEL
type
Thesis

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