Item Details

title

AUTOMATION OF CALIBRATION METHODS APPLIED TO INDUCTIVELY COUPLE PLASMA OPTICAL EMISSION SPECTROMETRY

author

Ingham, Jesse R.

abstract

Traditional calibration strategies, such as external standard calibration (EC), internal standardization (IS), and standard additions (SA), are widely utilized in quantitative analyses. Many samples present several concomitant species that can cause matrix effects, which can compromise the accuracy of an analytical method when EC or IS are applied. SA corrects for severe matrix effects; however, it involves extensive and time-consuming solution preparation and analyses. Thus, more efficient calibration approaches are required to achieve fast and accurate analytical methods.An example of a recent advancement in calibration is the standard dilution analysis method (SDA). SDA uses only two calibration solutions per sample, and it is as accurate as SA. Although SDA and some traditional calibration methods such as EC have proven themselves in a variety of applications, they still lack efficient automation of their sample preparation and analysis steps, which decreases productivity. In this dissertation, I describe the development of two strategies to further improve the efficiency of SDA, as well as a new approach to automate EC for inductively coupled plasma optical emission spectrometry (ICP-OES) applications. A switching valve that is a native feature on newer ICP-OES instruments is used for automation of both SDA and EC. A peristaltic pump stopping step and a multi-signal, gradient dilution approach are also explored for further automating SDA and EC, respectively. ICP-OES is the workhorse of any atomic spectrometry laboratory. It is a well-established analytical method that is applied to a wide variety of samples and in many fields, e.g. environmental, clinical, food, materials, etc. Therefore, calibration method improvements have the highest impact when developed for ICP-OES application. The strategies described in this dissertation are fast, accurate and green alternatives to traditional calibration methods. No external hardware or instrument modification are required for their implementation in routine analysis. They generate less waste and require fewer resources (i.e. reagents, gases, etc.) compared to traditional calibration strategies, which is amplified by their application in ICP-OES.

subject

Automation

Calibration

Gradient Dilution

ICP-OES

Method Development

contributor

Jones, Bradley T. (advisor)

Calloway, Clifton P. (committee member)

King, Bruce (committee member)

Ding, Wendu (committee member)

Hinze,, Willie L. (committee member)

date

2024-05-23T08:35:53Z (accessioned)

2024-05-23T08:35:53Z (available)

2024 (issued)

degree

Chemistry (discipline)

identifier

http://hdl.handle.net/10339/109402 (uri)

language

en (iso)

publisher

Wake Forest University

type

Dissertation