Item Details

title

APTAMER-BASED BIOSENSOR PLATFORM FOR CONTINUOUS, REAL-TIME MONITORING OF PROTEINS

author

Cheng, Joshua

abstract

The ability to monitor cell-secreted compounds in real-time is a key area of interest for organoid platforms. Currently practiced single time-point offline assays only offer delayed information and lack dynamic measurements needed to monitor rapid changes in cellular health and performance over time. Antibody-based sensors have been used frequently; however, their utility is hampered by limited uses due to the harsh conditions required to remove the target from the antibodies, requiring the sensor to be re-constructed before every measurement. In this study, we have developed an electrochemical aptamer-based biosensing platform for the continuous, real-time detection of human serum albumin secreted from liver cells. Aptamers are single-stranded DNA that binds in a highly specific and reversible manner and can dynamically track the concentration of a target in real-time. Our platform combines a novel durable and antifouling sensing layer with drift-resistant electrochemical methods to create a sensing platform optimized for continuous operation while integrated in cell culture. The sensors were able to continuously monitor proteins in real-time over a period of days and track dynamic changes in living cells. Furthermore, this platform is expanded to monitor several different analytes from a multi-organoid system. This biosensor platform offers an easily implemented, highly flexible means of monitoring organoid function in real time.

subject

aptamers

biosensors

electrochemical sensors

organ on a chip

contributor

Atala, Anthony (advisor)

Lee, Sang Jin (committee member)

Soker, Shay (committee member)

Yoo, James (committee member)

date

2024-05-23T08:36:16Z (accessioned)

2024 (issued)

degree

Biomedical Engineering (discipline)

embargo

2026-05-22 (terms)

2026-05-22 (liftdate)

identifier

http://hdl.handle.net/10339/109433 (uri)

language

en (iso)

publisher

Wake Forest University

type

Thesis