Item Details

title

Photoacoustic Imaging Assessment of Skeletal Muscle Oxygenation and Blood Perfusion with Doxorubicin-Induced Cardiotoxicity and Exercise Intervention

author

Harbert, Shona

abstract

Doxorubicin, a commonly used cancer chemotherapeutic agent, is cardiotoxic and causes deterioration in the left ventricle ejection fraction (LVEF) and fatigue. Exercise intervention has been suggested to ameliorate doxorubicin-induced cardiac decline, but further investigation of the relationship between doxorubicin, exercise intervention, LVEF, and fatigue is necessary. We implemented a mouse model of cancer treatment-induced cardiotoxicity across four animal cohorts: saline and sedentary (SAL SED), saline and exercise (SAL EX), doxorubicin and sedentary (DOX SED), and doxorubicin and exercise (DOX EX). The DOX SED cohort demonstrated a significant LVEF decline compared to the saline cohort controls while the DOX EX cohort ameliorated this decline. We conducted photoacoustic imaging on the skeletal muscle and found that skeletal muscle oxygen saturation in the DOX SED cohort significantly decreased compared to the saline cohorts. Skeletal muscle oxygen saturation in the DOX EX cohort did not significantly improve compared to the DOX SED cohort. Skeletal muscle total hemoglobin significantly increased in the DOX EX cohort compared to all the other cohorts. This study demonstrates that exercise intervention ameliorates doxorubicin-induced cardiotoxicity, and that more needs to be investigated about exercise intervention and chemotherapy-induced skeletal muscle wasting, fatigue, and changes in blood perfusion.

subject

Blood Perfusion

Cardiotoxicity

Doxorubicin

Exercise

Oxygenation

Photoacoustic Imaging

contributor

Weis, Jared A (advisor)

Meléndez, Giselle (committee member)

Kramer, Philip (committee member)

date

2025-06-24T08:36:40Z (accessioned)

2025 (issued)

degree

Biomedical Engineering (discipline)

embargo

2027-06-23 (terms)

2027-06-23 (liftdate)

identifier

http://hdl.handle.net/10339/111052 (uri)

language

en (iso)

publisher

Wake Forest University

type

Thesis