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Characterizing Changes in T Cell Composition and Function Within Prostate Tumors Following Androgen Ablation and Non-invasive Imaging of Anti-Tumor Immune Responses

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title
Characterizing Changes in T Cell Composition and Function Within Prostate Tumors Following Androgen Ablation and Non-invasive Imaging of Anti-Tumor Immune Responses
author
Akins, Elizabeth
abstract
Prostate cancer is the second leading cause of cancer deaths in American men and accounts for approximately 25% of all new cancer diagnoses. Currently, there is no effective therapy for the androgen independent disease that inevitably arises following androgen ablation therapy. The goal of cancer immunotherapy is to use the cells of the immune system to eradicate tumors. This is an especially attractive option for the treatment of androgen independent prostate cancer because as an immunogenic, non-vital organ, collateral prostate tissue damage is not life threatening. We set out to characterize the prostate infiltrating lymphocytes following androgen ablation as well as at later time points using standard immunohistochemical techniques. This method allowed us to determine not only localization of cells to the organ, but also whether the cells gain access to the tumor epithelium. While IHC is a very useful tool, we recognize the value of having an instantaneous and responsive means of monitoring the dynamic nature of immune responses. Molecular imaging is a method of detecting cells or cellular processes non-invasively within whole, living animals. This methodology has the potential to contribute significantly to the study of immunotherapy in preclinical models as well as in patients. Here we not only characterize the T cell localization and function in PTEN knockout prostate tumors, but also demonstrate the usefulness and feasibility of using molecular imaging as a tool to complement in vitro and ex vivo analysis of anti-tumor immune responses.
subject
tumor immunology
contributor
Willingham, Mark (committee chair)
Miller, Mark (committee member)
Kulik, George (committee member)
High, Kevin (committee member)
Dubey, Purnima (committee member)
date
2009-08-07T14:18:38Z (accessioned)
2010-06-18T18:59:40Z (accessioned)
2009-08-07T14:18:38Z (available)
2010-06-18T18:59:40Z (available)
2009-08-07T14:18:38Z (issued)
degree
Molecular Medicine (discipline)
identifier
http://hdl.handle.net/10339/14876 (uri)
language
en_US (iso)
publisher
Wake Forest University
rights
Release the entire work immediately for access worldwide. (accessRights)
type
Dissertation

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