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The role of auxin and ethylene in adventitious root formation in Arabidopsis and tomato

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abstract
Adventitious roots emerge from aerial plant tissues. Although important for clonal propagation of commercially important crop species, few studies have explored the mechanisms driving the development of these roots. This thesis research explored the hormonal controls and molecular mechanisms of adventitious root formation in Arabidopsis thaliana (Arabidopsis) and Solanum lycopersicum (tomato). Removal of the hypocotyl base and root from Arabidopsis seedlings enhanced the frequency of adventitious root formation. ACC treatment and mutations that cause enhanced ethylene synthesis reduced adventitious root formation in Arabidopsis, but enhanced adventitious root formation in tomato, with opposite effects found in ethylene insensitive mutations. These results are consistent with ethylene oppositely regulating adventitious root formation in these two species, while auxin has a similar stimulatory effect on adventitious root formation in both. Root excision increases both adventitious root formation and auxin transport. Additionally, local increases in auxin induced reporter expression after excision precede adventitious root formation and predict the position of root formation. These results indicate that local auxin accumulation due to changing transport may drive adventitious root formation. Moreover, the auxin transport proteins ABCB19 and PIN1, are required for efficient adventitious root formation. Transcript levels of PIN1 increased with root excision in hypocotyls, while neither ABCB19 transcripts nor pABCB19:GFP fluorescence was found to change. In contrast, pABCB19
subject
Auxin
Ethylene
adventitious roots
Hormonal cross talk
contributor
Sukumar, Poornima (author)
Winkel, Brenda SJ (committee chair)
Muday, Gloria K (committee member)
Tague, Brian (committee member)
Fahrbach, Susan (committee member)
McCauley, Anita (committee member)
date
2010-05-05T14:05:02Z (accessioned)
2010-06-18T18:59:49Z (accessioned)
2010-05-05T14:05:02Z (available)
2010-06-18T18:59:49Z (available)
2010-05-05T14:05:02Z (issued)
degree
Biology (discipline)
identifier
http://hdl.handle.net/10339/14894 (uri)
language
en_US (iso)
publisher
Wake Forest University
rights
Release the entire work for access only to the Wake Forest University system for one year from the date below. After one year, release the entire work for access worldwide. (accessRights)
title
The role of auxin and ethylene in adventitious root formation in Arabidopsis and tomato
type
Dissertation

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