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The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana

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abstract
Auxin transport polarity is established and maintained by the asymmetrical localization of auxin transport proteins in the plasma membrane. The SCD1 (stomata cytokinesis defective) gene encodes a protein containing a DENN domain, which is predicted to function as a RAB-GEF, which may be important in membrane protein targeting. The scd1-1 allele is a temperature sensitive mutant with a root phenotype evident at 25° C, but not at 18° C. The localization of auxin transport proteins, amount of auxin transport, and dependent physiological processes were examined in scd1-1. Basipetal or shootward auxin transport, and the dependent process of differential gravitropic growth, was lower in the scd1-1 mutant than wild type at 25° C, but not at 18° C. Laser scanning confocal microscopy was used to examine the localization of auxin transport proteins PIN1, PIN2, PIN3, MDR1, and AUX1 fused to fluorescent proteins. The PIN protein reporters were expressed in fewer cells at lower intensity in scd1-1 at 25° C. PIN1 and PIN2 exhibited altered localization and accumulation in endomembrane bodies. The endomembranes that accumulated PIN2
subject
auxin
DENN
gravitropism
PIN2
RAB-GEF
SCD1
contributor
Isley, Jonathan Wayne (author)
Muday, Gloria K (committee chair)
Tague, Brian W (committee member)
Fahrbach, Susan E (committee member)
date
2011-07-14T20:35:52Z (accessioned)
2012-07-14T08:30:17Z (available)
2011 (issued)
degree
Biology (discipline)
embargo
2012-07-14 (terms)
identifier
http://hdl.handle.net/10339/33467 (uri)
language
en (iso)
publisher
Wake Forest University
title
The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana
type
Thesis

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