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FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE

Electronic Theses and Dissertations

title: FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE
author: Suryadi, Jimmy
abstract: Our goal is to elucidate long range intramolecular signaling mechanisms in Escherichia coli methionyl tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.
subject: Fluorescence; MetRS; Pre-steady state kinetics; Synthetase; tRNA; Unnatural amino acid
contributor: Alexander, Rebecca W (committee chair); Bierbach, Ulrich (committee member); Colyer, Christa L (committee member); Comstock, Lindsay R (committee member)
date: 2011-07-14T20:35:54Z (accessioned); 2012-07-14T08:30:17Z (available); 2011 (issued)
degree: Chemistry (discipline)
embargo: 2012-07-14 (terms)
identifier: http://hdl.handle.net/10339/33470 (uri)
language: en (iso)
publisher: Wake Forest University
type: Dissertation