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FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE

Electronic Theses and Dissertations

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abstract
Our goal is to elucidate long range intramolecular signaling mechanisms in Escherichia coli methionyl tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.
subject
Fluorescence
MetRS
Pre-steady state kinetics
Synthetase
tRNA
Unnatural amino acid
contributor
Suryadi, Jimmy (author)
Alexander, Rebecca W (committee chair)
Bierbach, Ulrich (committee member)
Colyer, Christa L (committee member)
Comstock, Lindsay R (committee member)
date
2011-07-14T20:35:54Z (accessioned)
2012-07-14T08:30:17Z (available)
2011 (issued)
degree
Chemistry (discipline)
embargo
2012-07-14 (terms)
identifier
http://hdl.handle.net/10339/33470 (uri)
language
en (iso)
publisher
Wake Forest University
title
FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE
type
Dissertation

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