Item Details

abstract

Angiogenesis, the formation of new vessels from existing blood vessels, is essential for such physiological processes as wound healing and tumor growth. It is a highly regulated process, as numerous endogenous angiogenic modulators exist that both positively and negatively control vascularization. When the balance of modulators is altered, pathologic angiogenesis can occur. One such modulator is derived from high molecular weight kininogen, or HK, a plasma protein involved in the intrinsic blood coagulation pathway. Once HK is cleaved by kallikrein or another protease, HKa is produced, which is capable of reversibly binding to endothelial cells in the presence of Zn2+ and inhibiting angiogenesis. This action has been narrowed to the 109–amino acid domain 5 (HK5), a functional domain that binds to numerous endothelial cell surface receptors. By binding to domains 2 and 3 of the urokinase–type plasminogen activator receptor (uPAR), HK5 disrupts the interaction between uPAR and vitronectin, which activates the uPAR signaling pathway. Inhibiting the vitronectin–uPAR interaction causes an inhibition of endothelial cell adhesion, migration, tube formation and proliferation as well as inducing apoptosis. The minimum region of HK5 needed to induce antiangiogenic effects is a 29 residue stretch at the C–terminal end that has similar effects to HK5. The HK5 domain also binds to ferritin, an iron storage protein, which can inhibit the antiangiogenic effects of HK5. How ferritin inhibits the antiangiogenic effects of HK5 remains an important question.

subject

angiogenesis

biochemistry

ferritin

high molecular weight kininogen

protein-protein interactions

structural biology

contributor

Huhn, Annissa J. (author)

Hollis, Thomas (committee chair)

Torti, Suzy V (committee member)

Lowther, W. Todd (committee member)

Lyles, Douglas S (committee member)

Metheny-Barlow, Linda J (committee member)

date

2013-01-09T09:35:18Z (accessioned)

2013-07-09T08:30:10Z (available)

2012 (issued)

degree

Biochemistry and Molecular Biology (discipline)

embargo

2013-07-09 (terms)

identifier

http://hdl.handle.net/10339/37668 (uri)

language

en (iso)

publisher

Wake Forest University

title

Elucidating the Ferritin-High Molecular Weight Kininogen Domain 5 Interaction

type

Dissertation