Item Details

title

E1B and E4 adenoviral oncoproteins modulate cellular responses to DNA damage

author

Turner, Roberta Lynn

abstract

A productive adenovirus infection inundates the host cell with linear, double-stranded DNA molecules and an abundance of single-stranded DNA. The cellular responses of cell death and cell cycle arrest to this apparent profusion of damaged DNA are antagonized by overlapping activities encoded in the adenoviral E1B and E4 early genes. A mutant group C adenovirus that fails to express the E1B-55K and E4ORF3 genes is unable to suppress the DNA-damage response. Cells infected with this double-mutant virus display significant morphological heterogeneity at late times of infection. Fragmented nuclei and mitotic arrest frequently contribute to this heterogeneity.

subject

Adenovirus

AIF

cell cycle

cell death

DNA damage

oncolytic virus

contributor

Ornelles, David A (committee chair)

Lyles, Douglas S (committee member)

Alexander-Miller, Martha (committee member)

Parks, Griffith D (committee member)

Wilkinson, John C (committee member)

date

2014-01-15T09:35:34Z (accessioned)

2014-01-15T09:35:34Z (available)

2013 (issued)

degree

Microbiology & Immunology (discipline)

identifier

http://hdl.handle.net/10339/39131 (uri)

language

en (iso)

publisher

Wake Forest University

type

Dissertation