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E1B and E4 adenoviral oncoproteins modulate cellular responses to DNA damage

Electronic Theses and Dissertations

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abstract
A productive adenovirus infection inundates the host cell with linear, double-stranded DNA molecules and an abundance of single-stranded DNA. The cellular responses of cell death and cell cycle arrest to this apparent profusion of damaged DNA are antagonized by overlapping activities encoded in the adenoviral E1B and E4 early genes. A mutant group C adenovirus that fails to express the E1B-55K and E4ORF3 genes is unable to suppress the DNA-damage response. Cells infected with this double-mutant virus display significant morphological heterogeneity at late times of infection. Fragmented nuclei and mitotic arrest frequently contribute to this heterogeneity.
subject
Adenovirus
AIF
cell cycle
cell death
DNA damage
oncolytic virus
contributor
Turner, Roberta Lynn (author)
Ornelles, David A (committee chair)
Lyles, Douglas S (committee member)
Alexander-Miller, Martha (committee member)
Parks, Griffith D (committee member)
Wilkinson, John C (committee member)
date
2014-01-15T09:35:34Z (accessioned)
2014-01-15T09:35:34Z (available)
2013 (issued)
degree
Microbiology & Immunology (discipline)
identifier
http://hdl.handle.net/10339/39131 (uri)
language
en (iso)
publisher
Wake Forest University
title
E1B and E4 adenoviral oncoproteins modulate cellular responses to DNA damage
type
Dissertation

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