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Using Capillary Electrophoresis for High-Throughput Molecular Screening

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title
Using Capillary Electrophoresis for High-Throughput Molecular Screening
author
Riley, Kathryn Rosa
abstract
Advances in the development of DNA-encoded chemical libraries have necessitated rapid selection strategies to aid in molecular discovery. Encoded chemical libraries are composed of thousands of unique (potential) drug molecules, each encoded with a corresponding unique DNA sequence, allowing for the simultaneous screening of ligands against an identified biomolecular target. The basic principles driving DNA-encoded drug selection are mimicked by aptamer selection principles, which involve the partitioning of target-bound DNA from unbound DNA and the subsequent amplification of aptamers by PCR (polymerase chain reaction). Several screening methodologies have been proposed, including systematic evolution of ligands by exponential enrichment (SELEX) and nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). However, these stand-alone techniques require multiple rounds of selection and produce a limited number of each unique aptamer. SELEX has the added disadvantage of heavy resource consumption. Thus, it still remains to develop a fast, efficient method for high-throughput screening of encoded chemical libraries.
subject
capillary transient isotachophoresis (ctITP)
molecular selection
next generation sequencing (NGS)
SYBR Gold
contributor
Colyer, Christa L (committee chair)
Hantgan, Roy R (committee member)
Bierbach, Ulrich (committee member)
Hinze, Willie L (committee member)
Welker, Mark E (committee member)
date
2015-01-21T09:35:18Z (accessioned)
2016-01-21T09:30:16Z (available)
2014 (issued)
degree
Chemistry (discipline)
embargo
2016-01-21 (terms)
identifier
http://hdl.handle.net/10339/47456 (uri)
language
en (iso)
publisher
Wake Forest University
type
Dissertation

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