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Design of an In-House Insulin ELISA

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abstract
Insulin is a hormone synthesized by beta cells in the pancreas. In order to quantify the amount of insulin produced by the beta cells, different assays can be used to measure the concentration of insulin. Such assays include ELISAs and RIA. Both of these assays are used in various labs for measuring hormones including insulin. A few drawbacks to the RIA are that the assay takes two days to perform and more importantly, involves the use of radioactive materials. For these reasons, there is significant interest in developing an in-house insulin ELISA that will be faster in generating data than the RIA, be just as sensitive, while being about half of the cost of a commercial ELISA, and involve no radioactive materials.
subject
Antibody
Diabetes
ELISA
Immunoassay
Insulin
RIA
contributor
Enck, Kevin Mason (author)
Opara, Emmanuel C (committee chair)
Skardal, Aleks (committee member)
date
2016-01-11T09:35:20Z (accessioned)
2016-01-11T09:35:20Z (available)
2015 (issued)
degree
Biomedical Engineering (discipline)
identifier
http://hdl.handle.net/10339/57427 (uri)
language
en (iso)
publisher
Wake Forest University
title
Design of an In-House Insulin ELISA
type
Thesis

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