Item Details

title

Design of an In-House Insulin ELISA

author

Enck, Kevin Mason

abstract

Insulin is a hormone synthesized by beta cells in the pancreas. In order to quantify the amount of insulin produced by the beta cells, different assays can be used to measure the concentration of insulin. Such assays include ELISAs and RIA. Both of these assays are used in various labs for measuring hormones including insulin. A few drawbacks to the RIA are that the assay takes two days to perform and more importantly, involves the use of radioactive materials. For these reasons, there is significant interest in developing an in-house insulin ELISA that will be faster in generating data than the RIA, be just as sensitive, while being about half of the cost of a commercial ELISA, and involve no radioactive materials.

subject

Antibody

Diabetes

ELISA

Immunoassay

Insulin

RIA

contributor

Opara, Emmanuel C (committee chair)

Skardal, Aleks (committee member)

date

2016-01-11T09:35:20Z (accessioned)

2016-01-11T09:35:20Z (available)

2015 (issued)

degree

Biomedical Engineering (discipline)

identifier

http://hdl.handle.net/10339/57427 (uri)

language

en (iso)

publisher

Wake Forest University

type

Thesis