Home WakeSpace Scholarship › Electronic Theses and Dissertations

Biosynthesis and Functions of tRNA 2-Thiouridine in Bacillus subtilis

Electronic Theses and Dissertations

Item Files

Item Details

abstract
Cysteine desulfurases are PLP-dependent enzymes that catalyze the abstraction of sulfur from the free amino acid cysteine in a majority of organisms. Together with their sulfur acceptor proteins, these enzymes are required for the mobilization of sulfur for its incorporation into a wide variety of sulfur-containing biomolecules, including [Fe-S] clusters, thiamine, biotin and thionucleosides, such as 2-thiouridine. Thiolation of the wobble uridine on tRNA molecules to yield 2-thiouridine is critical for accuracy and efficiency in translation in all three domains of life, and depletion of this thionucleoside is associated with severe growth defects. Though its biosynthesis and functions have been well characterized in eukaryotes and Gram-negative bacteria, until now, little was known about its role or formation in Gram-positive bacteria.
subject
2-thiouridine
cysteine desulfurase
sulfur metabolism
thiocofactor
thioredoxin
tRNA modification
contributor
Black, Katherine Black (author)
Dos Santos, Patricia C (committee chair)
Furdui, Cristina M (committee member)
Alexander, Rebecca W (committee member)
Nelson, Kimberly J (committee member)
Welker, Mark E (committee member)
date
2017-01-14T09:35:30Z (accessioned)
2016 (issued)
degree
Chemistry (discipline)
2021-12-30 (liftdate)
embargo
2021-12-30 (terms)
identifier
http://hdl.handle.net/10339/64193 (uri)
language
en (iso)
publisher
Wake Forest University
title
Biosynthesis and Functions of tRNA 2-Thiouridine in Bacillus subtilis
type
Dissertation

Usage Statistics