Item Details

abstract

The purpose of this study was to investigate the regulation of SAMHD1 and its relation to nucleotide metabolism and cell cycle progression. Phosphorylation of SAMHD1 is the most extensively documented means of regulation. While questions still remain regarding regulation of SAMHD1 by phosphorylation, this work investigates an alternative mechanism of regulation. A major signaling pathway that has not been investigated with regards to SAMHD1 regulation is redox biology. Redox biology is emerging as a central regulator in myriad cellular processes including cell cycle progression, cellular metabolism, and cell signaling. Given the role that nucleotide metabolism plays in these processes, this study was undertaken to test the hypothesis that SAMHD1 is a redox regulated enzyme and that redox signaling is important for modulating SAMHD1 activity in vivo.

subject

Aicardi-Goutieres

cell cycle

deoxynucleotide triphosphate

HIV

Redox

SAMDH1

contributor

Mauney, Christopher Holbrook (author)

Hollis, Thomas J (committee chair)

Alexander, Rebecca (committee member)

Lowther, W. Todd (committee member)

Lyles, Doug (committee member)

Ornelles, David (committee member)

Perrino, Fred (committee member)

date

2017-06-15T08:36:12Z (accessioned)

2019-06-14T08:30:14Z (available)

2017 (issued)

degree

Biochemistry and Molecular Biology (discipline)

embargo

2019-06-14 (terms)

identifier

http://hdl.handle.net/10339/82241 (uri)

language

en (iso)

publisher

Wake Forest University

title

AN INVESTIGATION OF THE REDOX REGULATION OF THE DEOXYNUCLEOTIDE TRIPHOSPHOHYDROLASE SAMHD1 AND ITS ROLE IN NUCLEOTIDE HOMEOSTASIS

type

Dissertation