Item Details

abstract

The repair potential of the mammalian bladder has been known for over a century, but very few studies have examined the extent of bladder regeneration using animal models. The aim of the present studies was to develop a model of bladder regeneration in mice with specific emphasis on restoration of function. The impacts of immune response and a permissive microenvironment were also evaluated. Removal of a large portion of the urinary bladder (subtotal cystectomy; STC) in adult mice initiated a proliferative response that eventually gave rise to normal bladder wall architecture, including nerves, vessels, urothelium, and smooth muscle. Regenerated bladders displayed a normal low pressure, high capacity function, and were able to empty completely 12 weeks after cystectomy. Magnetic Resonance Imaging revealed a progressive increase in the bladder capacity and was able to predict the rate of bladder growth that resulted in normal function. InCITE, a combined optical tissue clearing method and computational analysis approach enabled a qualitative and quantitative exploration of the relationship between smooth muscle cell and extracellular matrix fiber morphometrics and functional recovery during repair.

subject

contributor

Zarifpour, Mona (author)

Marini, Frank (committee chair)

Hall, Adam (committee member)

Almedia Porada, Graca (committee member)

Christ, George (committee member)

date

2017-08-22T08:35:19Z (accessioned)

2019-08-21T08:30:10Z (available)

2017 (issued)

degree

Physiology and Pharmacology (discipline)

embargo

2019-08-21 (terms)

identifier

http://hdl.handle.net/10339/86332 (uri)

language

en (iso)

publisher

Wake Forest University

title

ESTABLISHING A MODEL OF ORGAN REGENERATION IN THE YOUNG MAMMAL: Manipulating and Developing a Permissive Microenvironment

type

Dissertation