Item Details

abstract

Nanopore sensing technology was initiated with the ultimate goal of developing a rapid, single-molecule sequencer. With the release of Oxford Nanopore Technology’s Minion sensor in 2014, that goal has been achieved. While this device utilizes biological nanopores, Solid-State Nanopore sensors were also initially investigated to develop sequencing devices. Significant efforts are being made to this day towards this end, with advances and improvements being made to amplifiers and using various 2-D materials for devices such Graphene, and MoS2. Other embodiments include, plasmonic nanopore devices, or integration of tunneling electrodes with SS-Nanopores. However, sequencing is not the only application that can be addressed by SS-nanopores, these devices have tremendous potential to detect various types of disease biomarkers such as nucleic acids, epigenetic modifications and proteins. Several studies have made significant efforts to develop novel assays to detect biological markers of disease such as cancer, and infectious disease agents. However, most of these involve significant interpretation of the electronic current signature, which can be difficult due to minor structural differences between analytes.

subject

Biomarkers

Epigenetics

Nanopores

Nucleic Acids

Single-molecule detection

contributor

Zahid, Osama (author)

Hall, Adam R (committee chair)

Howard, Timothy D (committee member)

Erie, Dorothy A (committee member)

Lyles, Douglas S (committee member)

Taylor, Ethan W (committee member)

Guthold, Martin (committee member)

date

2018-01-17T09:35:23Z (accessioned)

2019-01-16T09:30:08Z (available)

2017 (issued)

degree

Molecular Genetics & Genomics (discipline)

embargo

2019-01-16 (terms)

identifier

http://hdl.handle.net/10339/89870 (uri)

language

en (iso)

publisher

Wake Forest University

title

Detection of Nucleic Acid Disease Biomarkers Using Solid-State Nanopores

type

Dissertation