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Detection of Nucleic Acid Disease Biomarkers Using Solid-State Nanopores

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title
Detection of Nucleic Acid Disease Biomarkers Using Solid-State Nanopores
author
Zahid, Osama
abstract
Nanopore sensing technology was initiated with the ultimate goal of developing a rapid, single-molecule sequencer. With the release of Oxford Nanopore Technology’s Minion sensor in 2014, that goal has been achieved. While this device utilizes biological nanopores, Solid-State Nanopore sensors were also initially investigated to develop sequencing devices. Significant efforts are being made to this day towards this end, with advances and improvements being made to amplifiers and using various 2-D materials for devices such Graphene, and MoS2. Other embodiments include, plasmonic nanopore devices, or integration of tunneling electrodes with SS-Nanopores. However, sequencing is not the only application that can be addressed by SS-nanopores, these devices have tremendous potential to detect various types of disease biomarkers such as nucleic acids, epigenetic modifications and proteins. Several studies have made significant efforts to develop novel assays to detect biological markers of disease such as cancer, and infectious disease agents. However, most of these involve significant interpretation of the electronic current signature, which can be difficult due to minor structural differences between analytes.
subject
Biomarkers
Epigenetics
Nanopores
Nucleic Acids
Single-molecule detection
contributor
Hall, Adam R (committee chair)
Howard, Timothy D (committee member)
Erie, Dorothy A (committee member)
Lyles, Douglas S (committee member)
Taylor, Ethan W (committee member)
Guthold, Martin (committee member)
date
2018-01-17T09:35:23Z (accessioned)
2019-01-16T09:30:08Z (available)
2017 (issued)
degree
Molecular Genetics & Genomics (discipline)
embargo
2019-01-16 (terms)
identifier
http://hdl.handle.net/10339/89870 (uri)
language
en (iso)
publisher
Wake Forest University
type
Dissertation

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