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Behavioral aversion to AITC requires both painless and dTRPA1 in Drosophila

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abstract
There has been disagreement over the functional roles of the painless gene product in the detection and subsequent behavioral aversion to the active ingredient in wasabi, allyl isothiocyanate (AITC). Originally, painless was reported to eliminate the behavioral aversion to AITC, although subsequent reports suggested that another trpA homolog, dTRPA1, was responsible for AITC aversion. We re-evaluated the role of the painless gene in the detection of AITC, employing several different behavioral assays. Using the proboscis extension reflex (PER) assay, we observed that AITC did not reduce PER frequencies in painless or dTRPA1 mutants but did in wild-type genotypes. Quantification of food intake showed a significant decline in food consumption in the presence of AITC in wild-type, but not painless mutants.
subject
nociception
Drosophila
TRPA
allyl isothiocyanate
aversion
contributor
Mandel, S. (author)
Shoaf, M. (author)
Braco, J. (author)
Silver, W. (author)
Johnson, E. (author)
date
2020-03-09T16:15:46Z (accessioned)
2020-03-09T16:15:46Z (available)
7/3/18 (issued)
identifier
Mandel, S. J., Shoaf, M. L., Braco, J. T., Silver, W. L., & Johnson, E. C. J. (2018). Behavioral aversion to AITC requires both painless and dTRPA1 in Drosophila. Frontiers in neural circuits, 12, 45. (citation)
https://doi.org/10.3389/fncir.2018.00045 (doi)
http://hdl.handle.net/10339/96043 (uri)
language
en (iso)
publisher
Frontiers
rights
https://creativecommons.org/licenses/by/4.0/ (uri)
source
Frontiers in Neural Circuits
title
Behavioral aversion to AITC requires both painless and dTRPA1 in Drosophila
type
Article

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