Item Details

title

SYNBIOTIC YOGURT PREVENTS DIABETES PROGRESSION THROUGH IMPROVEMENTS IN THE MICROBIOME-INTESTINE-PANCREAS AXIS

author

Miller, Brandi Ciara

abstract

Type-2 diabetes mellitus (T2D) is a chronic disease characterized by increased blood glucose (hyperglycemia) and insulin resistance in peripheral metabolic organs, which hampers glucose clearance and leads to pancreatic beta cell dysfunction and reduced insulin secretion. Research has revealed the gut microbiome as a superlative regulator of metabolic health and the therapeutic effects of microbiome modulators, such as probiotics, prebiotics, and synbiotics, are emerging. Therefore, innovative and effective probiotics and/or prebiotics are in high demand to ameliorate diabetes. This study investigated the protective effects of a novel synbiotic yogurt (containing five human-origin Lactobacillus probiotics and a prebiotic isolated from sago) on the progression of T2D in a high-fat diet/streptozotocin-induced diabetic mouse model and compared its effects to those of a commercially-available yogurt. Synbiotic yogurt-fed mice exhibited improved fasting blood glucose and meal tolerance via protection of beta cells and intestinal physiology and favorable modulation of the gut microbiome. Surprisingly, mice fed the commercial yogurt demonstrated gut microbiome dysbiosis and damage to beta cells, which aggravated T2D progression. This study suggests that our novel synbiotic yogurt protects against diabetes, but raises concerns regarding the use of commercial yogurts in the progression of diabetes. Further studies are necessary to address these concerns.

subject

Gut microbiome

Metabolism

Prebiotics

Probiotics

Synbiotic yogurt

Type-2 diabetes mellitus

contributor

Yadav, Hariom (committee chair)

Solberg-Woods, Leah (committee member)

Yammani, Raghunatha (committee member)

date

2020-05-29T08:35:54Z (accessioned)

2021-05-28T08:30:12Z (available)

2020 (issued)

degree

Biomedical Science – MS (discipline)

embargo

2021-05-28 (terms)

identifier

http://hdl.handle.net/10339/96814 (uri)

language

en (iso)

publisher

Wake Forest University

type

Thesis