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OPTOGENETIC EXAMINATION OF POPULATION EXCITATION IN DEEP HIPPOCAMPAL CA1 PYRAMIDAL CELLS

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abstract
The brain exhibits many complex and emergent features that are attributed to its rhythmic activity. Oscillations of the brain are prevalent across global and local scales along a wide temporal spectrum, necessitating that investigations include both macro- and microscopic examination. Arguably, investigations into oscillatory mechanisms within local populations hold the most promise to inform cyclic regulation between network wide oscillations and synchronous firing of individual neurons. Given its unique recurrent connectivity and cellular organization, studies involving the hippocampus have uncovered much regarding the propagation and generation of brain rhythms. Particularly, they demonstrate the importance of regional selectivity in inducing cell-specific activation for precise information transfer and processing. The specific cells participating in oscillations is of paramount importance to selecting appropriate information types and processing procedures, which combine to produce the emergent functions of brain rhythms. Fortunately, recent advancements in optogenetics allow for such specificity. However, more detailed studies examining the role of each cell type are lacking. While a few studies employ optogenetics to induce oscillatory activity in vivo, even fewer have successfully employed such methods in vitro, a necessity for a comprehensive understanding. This work combines optogenetic methods with in vitro assessment of excitability within local populations. Through careful characterization of the Thy1 Line 18 mouse, ChR2 expression was found to exhibit subregional specificity with localized expression in the deep sublayer of CA1. A light intensity response curve (LIRC) was then generated with this transgenic line for the assessment of excitation within local
subject
Electrophysiology
Interneurons
Neuroscience
Optogenetics
Oscillations
Pharmacology
contributor
Dobbins, Dorothy (author)
Godqin, Dwayne W (committee chair)
Weiner, Jeffery (committee member)
Ma, Tao (committee member)
McCool, Brian (committee member)
Raab-Graham, Kimberly (committee member)
date
2020-08-28T08:35:31Z (accessioned)
2020 (issued)
degree
Neuroscience (discipline)
2022-08-27 (liftdate)
embargo
2022-08-27 (terms)
identifier
http://hdl.handle.net/10339/96957 (uri)
language
en (iso)
publisher
Wake Forest University
title
OPTOGENETIC EXAMINATION OF POPULATION EXCITATION IN DEEP HIPPOCAMPAL CA1 PYRAMIDAL CELLS
type
Dissertation

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