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DEVELOPMENT OF A PHASE CHANGE CONTRAST AGENT FOR DUAL FLUORINE-MRI ULTRASOUND SENTINEL LYMPH NODE ASSESSMENT IN PRIMARY BREAST CANCER

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abstract
Breast cancer continues to be the second leading cause of cancer-related death in women with over 40,000 deaths per year. Sentinel lymph node (SLN) biopsy is considered imperative to the staging of breast cancer and is the most useful prognostic indicator of cancer survival. Current SLN location methods have substantial limitations and SLN biopsy is relatively invasive with risk of complications. In this work, I propose a novel dual-contrast agent for ultrasound (US) and fluorine (19F) Magnetic Resonance Imaging (MRI) imaging of the local lymphatics for the purpose of locating and assessing the sentinel lymph node for metastasis with enhanced specificity and sensitivity. This work aims to first develop a decafluorobutane (DFB) nanodroplet(ND), imageable with 19F MRI and US, which can spontaneously change from liquid to gas phase upon US application. Since no LBPCCA, including DFB, has previously been imaged with MRI, full optimization was conducted. NDs were, for the first time, imaged with both MRI and US and characterized for size and stability. The second aim was to investigate the ability of DFB NDs to be taken up and retained by macrophages. Raw 264.7 cells were incubated with DFB NDs to investigate their ND uptake, retention, and imageability. The results of these studies for the first time demonstrate the utility of DFB as a phase change dual contrast agent for 19F MRI and US. In addition, these results show the potential of DFB NDs for cell tracking of macrophage migration into the lymphatics.
subject
Fluorine
MRI
Perfluorocarbon
Phase Change Contrast Agent
Sentinel Lymph Node
Ultrasound
contributor
Crowe, William (author)
Zhao, Dawen (committee chair)
Bourland, John D (committee member)
Chan, Michael D (committee member)
Jung, Youngkyoo (committee member)
Verbridge, Scott S (committee member)
date
2021-06-03T08:35:58Z (accessioned)
2021 (issued)
degree
Biomedical Engineering (discipline)
2021-12-02 (liftdate)
embargo
2021-12-02 (terms)
identifier
http://hdl.handle.net/10339/98793 (uri)
language
en (iso)
publisher
Wake Forest University
title
DEVELOPMENT OF A PHASE CHANGE CONTRAST AGENT FOR DUAL FLUORINE-MRI ULTRASOUND SENTINEL LYMPH NODE ASSESSMENT IN PRIMARY BREAST CANCER
type
Dissertation

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