Home WakeSpace Scholarship › Electronic Theses and Dissertations

OPTIMIZATION OF A MULTI-ORGANOID-CHIP AS A POTENTIAL TOOL FOR MODELING TOXICITY

Electronic Theses and Dissertations

Item Files

Item Details

abstract
Drug screening is a critical step in the development of effective and safe therapeutic agents. One key phase in the drug screening process is performing toxicity assays on the candidate drugs to assess their safety for the host. Toxicity assays are a costly and slow process. Furthermore, more than 80% of drugs fail in clinical testing. It is thus imperative to develop fast and cost-effective platforms for toxicity assays. Thus far, a few engineered platforms have been developed and used successfully for toxicity assays. However, these platforms use complex designs that are costly in terms of both material and human resources. This Master’s thesis investigated the fabrication and optimization of a relatively simple and inexpensive multi-organ-on-a-chip that could potentially be used as a platform for toxicity assays. The chip-based model system combined organoid technology with advanced microfluidics and housed three kinds of organoids: liver, colon, and lung. The chip design and development went through numerous iterations before it reached its final form, the V7. The V7 was a simple platform and was able to house the three organoid types. Week 1 images showed that the chip was able to maintain an uncontaminated environment that sustained the structural integrity of the organoids; however, the viability of the organoids was not satisfactory by the end of Week 2. We discuss possible reasons for these findings and provide recommendations for improving the V7.
subject
Chip
Intestine
Liver
Lung
Model
Oganoid
contributor
Lent, Nicholas C (author)
Bishop, Colin (committee chair)
Walker, Steve (committee member)
Reeves, Kimberly D (committee member)
Schwartz, Marshall Z (committee member)
date
2021-09-01T08:35:20Z (accessioned)
2021-09-01T08:35:20Z (available)
2021 (issued)
degree
Biomedical Science – MS (discipline)
identifier
http://hdl.handle.net/10339/99056 (uri)
language
en (iso)
publisher
Wake Forest University
title
OPTIMIZATION OF A MULTI-ORGANOID-CHIP AS A POTENTIAL TOOL FOR MODELING TOXICITY
type
Thesis

Usage Statistics