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oai:null:10339/334302026-09-02T14:33:05Zcom_10339_14934col_10339_38132

Armbruster, Chelsie Elizabeth 2011-07-14T20:35:09Z 2012-01-14T09:30:12Z 2011 https://wakespace.lib.wfu.edu/handle/10339/33430 Otitis media (OM) is among the most common childhood diseases worldwide. OM infections often persist for long periods of time and can be highly resistant to antibiotic treatment due in part to persistence of bacteria within biofilms. The majority of OM infections also involve coinfection by multiple species. For instance, the leading causative agents <italic>Haemophilus influenzae</italic> and <italic>Moraxella catarrhalis</italic> are more frequently found to coexist than to be isolated individually. Polymicrobial infection and biofilm formation may have a dramatic impact on the severity and treatability of OM. Thus, there is a pressing need to investigate polymicrobial infection and to identify factors that contribute to the establishment of these infections. One factor that may contribute to the establishment of polymicrobial infection is bacterial communication via quorum signaling. Autoinducer-2 (AI-2) is termed an interspecies signal as the genetic determinant for AI-2 production (<italic>luxS</italic>) is highly conserved and many species possess the machinery to respond to AI-2. <italic>H. influenzae</italic> has a <italic>luxS</italic> homolog whereas <italic>M. catarrhalis</italic> does not but may still sense and respond to AI-2. Therefore, the focus of this project was to investigate the impact of AI-2 intra- and interspecies bacterial communication and <italic>H. influenzae</italic> and <italic>M. catarrhalis</italic> polymicrobial infection on biofilm formation, antibiotic resistance, and persistence in experimental OM. en Wake Forest University Autoinducer 2 Biofilm Haemophilus influenzae Moraxella catarrhalis Otitis media Quorum sensing HAEMOPHILUS INFLUENZAE AND MORAXELLA CATARRHALIS INTRA- AND INTERSPECIES QUORUM SIGNALING Dissertation Swords, William E Shelness, Gregory Kock, Nancy Ornelles, David Reid, Sean Microbiology & Immunology 2012-01-14 open.access