2026-09-21T20:52:31Zhttps://wakespace.lib.wfu.edu/server/oai/request

oai:null:10339/334902026-09-02T16:52:32Zcom_10339_14934col_10339_38132

Krishnan, Bhavani 2011-07-14T20:36:20Z 2011 https://wakespace.lib.wfu.edu/handle/10339/33490 Ang-(1-7) is an endogenous peptide hormone that is produced in the circulation and in tissues by proteolytic cleavage of angiotensin I or II. The heptapeptide mediates biological responses by activating a unique G-protein-coupled angiotensin-(1-7) [AT(1-7)] receptor mas, thereby providing specific targeted actions when used as a therapeutic agent. In this study, athymic mice with human LNCaP xenografts were infused with saline or Ang-(1-7) (24 g/kg/h) for 54 days. The heptapeptide markedly reduced tumor volume and wet weight as compared to saline administration. Tumors from mice treated with Ang-(1-7) had a 78% reduction in Ki67 and a 55% decrease in the phosphorylation of the MAP kinases ERK1 and ERK2, compared to tumor tissue from Ang-(1-7)-medicated animals, suggesting that the heptapeptide reduces cell proliferation. A significant reduction in both LNCaP and PC3 cell growth was observed following incubation with 100 nM Ang-(1-7) in vitro, supporting the anti-proliferative properties of the heptapeptide. Mas was detected by Western blot hybridization in both cell types. en Wake Forest University Angiogenesis Angiotensin-(1-7) Metastasis Prostate Cancer sFlt-1 Vascular Endothelial Growth factor Angiotensin-(1-7) Reduces Prostate Cancer Growth and Metastasis by Altering the Tumor Microenvironment Dissertation Tallant, Elisabeth. Ann Dubey, Purnima Gallagher, Patricia E Lively, Mark O Daniel, Larry W Molecular Genetics & Genomics restricted