2026-09-20T00:44:15Zhttps://wakespace.lib.wfu.edu/server/oai/request

oai:null:10339/907102026-09-02T11:58:02Zcom_10339_14934col_10339_38132

Liu, Yin 2018-05-24T08:36:03Z 2019-05-23T08:30:13Z 2018 https://wakespace.lib.wfu.edu/handle/10339/90710 Breast cancer is one of major public health problem worldwide. However, the underlying molecular mechanism of breast cancer progression and metastasis is not well understood. Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer which could progress to or recur as invasive breast cancer. To identify the novel regulators of DCIS progression, we performed gene expression analysis of syngeneic cell lines MCF10A, DCIS.com and MCF10CA and cross-referenced the targets using patient cohorts. By comparing gene expression between MCF10A and DCIS.com cell line, we identified ID2 as a regulator of DCIS initiation. ID2 promoted DCIS formation by enhancing cancer stemness of premalignant cells. On the other hand, we compared the DCIS.com and invasive breast cancer cell line, MCF10CA, and identified INHBA and GJB2 as novel regulators of aggressive phenotype. These 2 genes regulated migration, colonization and stemness of invasive cancer cells. Finally, we identified an ID2 inhibitor, natural compound Helichrysetin. en Wake Forest University Breast cancer Cancer Metastasis Prognosis BREAST CANCER PROGRESSION AND METASTASIS: DISCOVERY OF NOVEL GENES AND THEIR FUNCTIONAL STUDY Dissertation Watabe, Kounosuke Lo, Hui-Wen Vidi, Pierre-Alexander Miller, Lance D Metheny-Barlow, Linda Cancer Biology 2019-05-23 open.access