Kinetic and mutational studies of two RNA-interacting enzymes
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- abstract
- Cellular enzymes interact with RNA substrates at several key stages in an organism's life cycle, particularly during the processes of transcription and translation. A more detailed description of enzyme mechanistic features and kinetic parameters is important both for understanding molecular function in general and as possible therapeutic targets. The two classes of RNA-interacting enzymes investigated in the work described here are helicases, which disrupt RNA secondary structure, and aminoacyl-tRNA synthetases, which catalyze the attachment of an amino acid to its cognate tRNA.
- subject
- Pre-steady state kinetics
- Prokaryote
- Protein biosynthesis
- RNA stability
- steady state kinetics
- strand displacement
- contributor
- Alexander, Rebecca W. (committee chair)
- Hollis, Thomas (committee member)
- Bierbach, Ulrich (committee member)
- Colyer, Christa (committee member)
- King, S. Bruce (committee member)
- date
- 2012-01-18T09:35:30Z (accessioned)
- 2011 (issued)
- degree
- Chemistry (discipline)
- 10000-01-01 (liftdate)
- embargo
- forever (terms)
- identifier
- http://hdl.handle.net/10339/36429 (uri)
- language
- en (iso)
- publisher
- Wake Forest University
- title
- Kinetic and mutational studies of two RNA-interacting enzymes
- type
- Dissertation