Home WakeSpace Scholarship › Electronic Theses and Dissertations

Kinetic and mutational studies of two RNA-interacting enzymes

Electronic Theses and Dissertations

Item Files

Item Details

title
Kinetic and mutational studies of two RNA-interacting enzymes
author
Casina, Veronica Christine
abstract
Cellular enzymes interact with RNA substrates at several key stages in an organism's life cycle, particularly during the processes of transcription and translation. A more detailed description of enzyme mechanistic features and kinetic parameters is important both for understanding molecular function in general and as possible therapeutic targets. The two classes of RNA-interacting enzymes investigated in the work described here are helicases, which disrupt RNA secondary structure, and aminoacyl-tRNA synthetases, which catalyze the attachment of an amino acid to its cognate tRNA.
subject
Pre-steady state kinetics
Prokaryote
Protein biosynthesis
RNA stability
steady state kinetics
strand displacement
contributor
Alexander, Rebecca W. (committee chair)
Hollis, Thomas (committee member)
Bierbach, Ulrich (committee member)
Colyer, Christa (committee member)
King, S. Bruce (committee member)
date
2012-01-18T09:35:30Z (accessioned)
2011 (issued)
degree
Chemistry (discipline)
embargo
forever (terms)
10000-01-01 (liftdate)
identifier
http://hdl.handle.net/10339/36429 (uri)
language
en (iso)
publisher
Wake Forest University
type
Dissertation

Usage Statistics